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Anti-Rac1 specific mouse MAb(sample size)

Anti-Rac1 specific mouse MAb(sample size)

Item no.	ARC03-S
Manufacturer	Cytoskeleton
Amount	1 x 25 ul
Quantity options	2 x 100 ul 2 x 50 ug 6 x 50 ug 1 x 25 ul
Category	Antibodies Primary Antibodies
Type	Antibody
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Shipping condition	Room temperature
Available
Shipping Temperature	AT
Storage Conditions	On Arrival: 4°C
Introduction	Rac1 is one of most extensively studied member of Rho GTPase family. This family is involved in a wide range of cellular responses, including cytoskeletal reorganization, regulation of transcription, cell migration, cellular transformation and metastasis. Rac1 is expressed in a large number of different cell types.
Associated Products	nti-RhoA monoclonal antibody (Cat.#ARH03) Anti-Cdc42 monoclonal antibody (Cat.#ACD03) Cdc42 G-LISAActivation Assay (Cat.#BK127) RhoA G-LISAActivation Assay (Cat.#BK124) Rac1 G-LISAActivation Assay (Cat.#BK126) Rac1, 2, 3 G-LISAActivation Assay (Cat.#BK125)
Weight (grams)	12
Product Uses	This antibody is recommended for detection of Rac1 in human, mouse, rat or other extracts. The following protocols have been tested with this antibody: Western blot: recommended Immunocytochemistry: recommended
Material	The anti-Rac1 antibody (Cat. # ARC03) is a mouse monoclonal antibody that only recognizes Rac1, not Rac2, Rac3, Cdc42 or other small GTPases. Other commercially available Rac1 antibodies recognize Rac2 and 3, some even recognize Cdc42 (Table 1 and Figure 1). Platelet extract (Cat. # EXT01) is included as a positive control. ARC03 is supplied as a lyophilized white powder.
Figure 1 Legend	Figure 1 legend - Western blot of purified Rac1, 2, 3, Cdc42 and platelet extracts probed with anti-Rac1 monoclonal antibody (Cat. # ARC03) and other commercially availabe Rac1 antibodies. The detection of 25 ng His-Rac1 ( lane 1), His-Rac2 (lane 2), GST-Rac3 (lane 3), GST-Cdc42 (lane 4) and 50 ug of platelet extract (lane 5). ARC03 does not cross-react with Rac2, 3 or Cdc42, while all other commercially available Rac1 antibody crossreact with GTPases other than Rac1. The blot was probed with a 1 ug/ml (1:500) dilution of ARC03. Other blots were probed according to manufacturers' protocols.
Figure 2 Legend	Figure 2 - Immunofluorescence image of mouse Swiss 3T3 cells stained with Anti-Rac1 monoclonal antibody (Cat. # ARC03). Swiss 3T3 cells were grown to semi-confluency and fixed with methanol. Immunofluorescence staining using 1 ug/ml (1:20 dilution) of ARC03 antibody is shown (red). The primary antibody was detected with a 1:500 dilution of goat anti-mouse rhodamine conjugated antibody. Photograph was taken with a 100X objective lens.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

ARC03-S.pdf

ARC03-S-datasheet.pdf

ARC03-S-safety-datasheet.pdf