Item no. |
CSB-RP057294h-200 |
Manufacturer |
Cusabio
|
Amount |
200ug |
Quantity options |
1mg
10ug
100ug
200ug
50ug
500ug
|
Category |
|
Type |
Proteins Recombinant |
Specific against |
other |
Host |
E.coli |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
Ribonuclease 5 |
Available |
|
Research Topic |
Signal Transduction |
Uniprot ID |
P03950 |
Gene Names |
RNASE5 |
Organism |
Homo sapiens (Human) |
AA Sequence |
DNSRYTHFLTQHYDAKPQGRDDRYCESIMRRRGLT SPCKDINTFIHGNKRSIKAICENKNGNPHRENLRI SKSSFQVTTCKLHGGSPWPPCQYRATAGFRNVVVA CENGLPVHLDQSIFRRP |
Expression Region |
26-147aa |
Sequence Info |
Partial |
Source |
E.coli |
Tag Info |
N-terminal 6xHis-tagged |
MW |
18 kDa |
Alternative Name(s) |
Ribonuclease 5 |
Relevance |
Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo. |
Reference |
"Diversifying selection of the tumor-growth promoter angiogenin in primate evolution." Zhang J., Rosenberg H.F. Mol. Biol. Evol. 19:438-445(2002) |
Purity |
Greater than 90% as determined by SDS-PAGE. |
Storage Buffer |
Tris-based buffer, 50% glycerol |
Storage |
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C. |
Notes |
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week. |
Function |
Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo. |
Involvement in disease |
Amyotrophic lateral sclerosis 9 (ALS9) |
Subcellular Location |
Cytoplasmic vesicle, secretory vesicle lumen, Secreted, Nucleus, Nucleus, nucleolus |
Protein Families |
Pancreatic ribonuclease family |
Tissue Specificity |
Expressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.