Recombinant Human Probable ATP-dependent RNA helicase DDX27(DDX27),partial

Epigenetics and Nuclear Signaling

DDX27

DNMLADLGLIGTIGEDDEVPVEPESDSGDEEEEGP IVLGRRQKALGKNRSADFNPDFVFTEKEGTYDGSW ALADVMSQLKKKRAATTLDEKIEKVRKKRKTEDKE AKSGKLEKEKEAKEGSEPKEQEDLQENDEEGSEDE ASETDYSSADENILTKADTLKVKDRKKKKKKGQEA GGFFEDASQYDENLSFQDMNLSRPLLKAITAMGFK QPTPIQKACIPVGLLGKDICACAATGTGKTAAFAL PVLERLIYKPRQAPVTRVLVLVPTRELGIQVHSVT RQLAQFCNITTCLAVGGLDVKSQEAALRAAPDILI ATPGRLIDHLHNCPSFHLSSIEVLILDEADRMLDE YFEEQMKEIIRMCSHHRQTMLFSATMTDEVKDLAS VSLKNPVRIFVNSNTDVAPFLRQEFIRIRPNREGD REAIVAALLTRTFTDHVMLFTQTKKQAHRMHILLG LMGLQVGELHGNLSQTQRLEALRRFKDEQIDILVA TDVAARGLDIEGVKTVINFTMPNTIKHYVHRVGRT ARAGRAGRSVSLVGEDERKMLKEIVKAAKAPVKAR ILPQDVILKFRDKIEKMEKDVYAVLQLEAEEKEMQ QSEAQINTAKRLLEKGKEAVVQEPERSWFQTKEER KKEKIAKALQEFDLALRGKKKRKKFMKDAKKKGEM TAEERSQFEILKAQMFAERLAKRNRRAKRARAMPE EEPVRGPAKKQKQGKKSVFDEELTNTSKKALKQYR AGPSFEERKQLGLPHQRRGGNFKSKSRYKR

Partial

N-terminal 6xHis-SUMO-tagged

DEAD box protein 27

Wang Y., Fan Y.-Z., Han W.-L., Yang T., Gao Y., Ma D.-L.The DNA sequence and comparative analysis of human chromosome 20.Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.Nature 414:865-871(2001)

Tris-based buffer, 50% glycerol

Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.

Nucleus, nucleolus, Chromosome

N-terminal 6xHis-SUMO-tagged