Item no. |
CSB-EP822281HU-100 |
Manufacturer |
Cusabio
|
Amount |
100ug |
Category |
|
Type |
Proteins Recombinant |
Format |
Liquid or Lyophilized powder |
Specific against |
other |
Host |
E.coli |
Conjugate/Tag |
GST |
Purity |
Greater than 90% as determined by SDS-PAGE. |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
Androgen receptor N-terminal-interacting protein,CH-rich-interacting match with PLAG1,E3 ubiquitin-protein ligase Pirh2,RING finger protein 199,Zinc finger protein 363,p53-induced RING-H2 protein |
Available |
|
Research Areas |
Cell Biology |
Uniprot ID |
Q96PM5 |
Gene Names |
RCHY1 |
Organism |
Homo sapiens (Human) |
AA Sequence |
MAATAREDGASGQERGQRGCEHYDRGCLLKAPCCD KLYTCRLCHDNNEDHQLDRFKVKEVQCINCEKIQH AQQTCEECSTLFGEYYCDICHLFDKDKKQYHCENC GICRIGPKEDFFHCLKCNLCLAMNLQGRHKCIENV SRQNCPICLEDIHTSRVVAHVLPCGHLLHRTCYEE MLKEGYRCPLCMHSALDMTRYWRQLDDEVAQTPMP SEYQNMTVDILCNDCNGRSTVQFHILGMKCKICES YNTAQAGGRRISLDQQ |
Expression Region |
1-261aa |
Sequence Info |
Full Length |
Source |
E.coli |
Tag Info |
N-terminal GST-tagged |
MW |
57.1 kDa |
Alternative Name(s) |
Androgen receptor N-terminal-interacting protein CH-rich-interacting match with PLAG1 E3 ubiquitin-protein ligase Pirh2 RING finger protein 199 Zinc finger protein 363 p53-induced RING-H2 protein |
Relevance |
Mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including p53/TP53, P73, HDAC1 and CDKN1B. Preferentially acts on tetrameric p53/TP53. Monoubiquitinates the translesion DNA polymerase POLH. Contributes to the regulation of the cell cycle progression. Increases AR transcription factor activity. |
Reference |
A novel hPirh2 splicing variant without ubiquitin protein ligase activity interacts with p53 and is down-regulated in hepatocellular carcinoma. Wu G., Sun M., Zhang L., Zhou J., Wang Y., Huo K. FEBS Lett. 584:2772-2778(2010) |
Purity |
Greater than 90% as determined by SDS-PAGE. |
Form |
Liquid or Lyophilized powder |
Buffer |
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Storage |
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C. |
Notes |
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week. |
Function |
Mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including p53/TP53, P73, HDAC1 and CDKN1B. Preferentially acts on tetrameric p53/TP53. Monoubiquitinates the translesion DNA polymerase POLH. Contributes to the regulation of the cell cycle progression. Increases AR transcription factor activity. |
Subcellular Location |
Nucleus, Nucleus speckle, Cytoplasm |
Paythway |
p53signalingpathway |
Tag Information |
N-terminal GST-tagged |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.