Comparison

Recombinant Arabidopsis thaliana NADPH-dependent oxidoreductase 2-alkenal reductase(AER)

Item no. CSB-EP654400DOA-1
Manufacturer Cusabio
Amount 1mg
Category
Type Proteins Recombinant
Format Liquid or Lyophilized powder
Specific against other
Host E.coli
Purity Greater than 90% as determined by SDS-PAGE.
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias Short name:DBR1
Available
Research Areas
Others
Uniprot ID
Q39172
Gene Names
P1
Organism
Arabidopsis thaliana (Mouse-ear cress)
AA Sequence
MTATNKQVILKDYVSGFPTESDFDFTTTTVELRVP EGTNSVLVKNLYLSCDPYMRIRMGKPDPSTAALAQ AYTPGQPIQGYGVSRIIESGHPDYKKGDLLWGIVA WEEYSVITPMTHAHFKIQHTDVPLSYYTGLLGMPG MTAYAGFYEVCSPKEGETVYVSAASGAVGQLVGQL AKMMGCYVVGSAGSKEKVDLLKTKFGFDDAFNYKE ESDLTAALKRCFPNGIDIYFENVGGKMLDAVLVNM NMHGRIAVCGMISQYNLENQEGVHNLSNIIYKRIR IQGFVVSDFYDKYSKFLEFVLPHIREGKITYVEDV ADGLEKAPEALVGLFHGKNVGKQVVVVARE
Expression Region
1-345aa
Sequence Info
Full Length
Source
E.coli
Tag Info
N-terminal 6xHis-SUMO-tagged
MW
54.1 kDa
Alternative Name(s)
Short name:DBR1
Relevance
Catalyzes the reduction of the 7-8 double bond of phenylpropanal substrates, such as p-coumaryl aldehyde and coniferyl aldehyde (in vitro). Has activity towards toxic substrates, such as 4-hydroxy-(2E)-nonenal (in vitro). May play a distinct role in plant antioxidant defense and is possibly involved in NAD(P)/NAD(P)H homeostasis.
Reference
Arabidopsis thaliana NADPH oxidoreductase homologs confer tolerance of yeasts toward the thiol-oxidizing drug diamide.Babiychuk E., Kushnir S., Belles-Boix E., van Montagu M., Inze D.J. Biol. Chem. 270:26224-26231(1995)
Purity
Greater than 90% as determined by SDS-PAGE.
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
Function
Involved in the detoxification of reactive carbonyls
Subcellular Location
Cytoplasm, Nucleus, nucleoplasm
Protein Families
NADP-dependent oxidoreductase L4BD family
Tissue Specificity
Expressed in leaves.
Tag Information
N-terminal 6xHis-SUMO-tagged

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 1mg
Available: In stock
available

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