Comparison

Recombinant Chlamydia pneumoniae Large cysteine-rich periplasmic protein OmcB(omcB),partial

Item no. CSB-EP326426DRZ-100
Manufacturer Cusabio
Amount 100ug
Category
Type Proteins Recombinant
Format Liquid or Lyophilized powder
Specific against other
Host E.coli
Purity Greater than 90% as determined by SDS-PAGE.
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias Large-CRP,Alternative name(s):,60KDA cysteine-rich OMP,Short name:,60KDA CRP,60KDA outer membrane protein,Cysteine-rich outer membrane protein
Available
Research Areas
Microbiology
Uniprot ID
P23700
Gene Names
omcB
Organism
Chlamydia pneumoniae (Chlamydophila pneumoniae)
AA Sequence
SAETKPAPVPMTAKKVRLVRRNKQPVEQKSRGAFC DKEFYPCEEGRCQPVEAQQESCYGRLYSVKVNDDC NVEICQSVPEYATVGSPYPIEILAIGKKDCVDVVI TQQLPCEAEFVSSDPETTPTSDGKLVWKIDRLGAG DKCKITVWVKPLKEGC
Expression Region
41-196aa
Sequence Info
Partial
Source
E.coli
Tag Info
N-terminal 6xHis-SUMO-tagged
MW
33.3 kDa
Alternative Name(s)
Large-CRP
Alternative name(s):
60KDA cysteine-rich OMP
Short name:
60KDA CRP
60KDA outer membrane protein
Cysteine-rich outer membrane protein
Relevance
In elementary bodies (EBs, the infectious stage, which is able to survive outside the host cell) provides the structural integrity of the outer envelope through disulfide cross-links with the small cysteine-rich protein and the major outer membrane porin. It has been described in publications as the Sarkosyl-insoluble COMC (Chlamydia outer membrane complex), and serves as the functional equivalent of peptidoglycan (By similarity).
Reference
Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA.Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K., Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T.Nucleic Acids Res. 28:2311-2314(2000).
Purity
Greater than 90% as determined by SDS-PAGE.
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
Function
In elementary bodies (EBs, the infectious stage, which is able to survive outside the host cell) provides the structural integrity of the outer envelope through disulfide cross-links with the small cysteine-rich protein and the major outer membrane porin. It has been described in publications as the Sarkosyl-insoluble COMC (Chlamydia outer membrane complex), and serves as the functional equivalent of peptidoglycan (By similarity).
Subcellular Location
Periplasm
Tag Information
N-terminal 6xHis-SUMO-tagged

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100ug
Available: In stock
available

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