Item no. |
CSB-CF006835HU-10 |
Manufacturer |
Cusabio
|
Amount |
10ug |
Category |
|
Type |
Proteins Recombinant |
Format |
Liquid or Lyophilized powder |
Specific against |
other |
Conjugate/Tag |
Myc |
Purity |
Greater than 85% as determined by SDS-PAGE. |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Available |
|
Research Topic |
Signal Transduction |
Uniprot ID |
P52429 |
Gene Names |
DGKE |
Organism |
Homo sapiens (Human) |
AA Sequence |
MEAERRPAPGSPSEGLFADGHLILWTLCSVLLPVF ITFWCSLQRSRRQLHRRDIFRKSKHGWRDTDLFSQ PTYCCVCAQHILQGAFCDCCGLRVDEGCLRKADKR FQCKEIMLKNDTKVLDAMPHHWIRGNVPLCSYCMV CKQQCGCQPKLCDYRCIWCQKTVHDECMKNSLKNE KCDFGEFKNLIIPPSYLTSINQMRKDKKTDYEVLA SKLGKQWTPLIILANSRSGTNMGEGLLGEFRILLN PVQVFDVTKTPPIKALQLCTLLPYYSARVLVCGGD GTVGWVLDAVDDMKIKGQEKYIPQVAVLPLGTGND LSNTLGWGTGYAGEIPVAQVLRNVMEADGIKLDRW KVQVTNKGYYNLRKPKEFTMNNYFSVGPDALMALN FHAHREKAPSLFSSRILNKAVYLFYGTKDCLVQEC KDLNKKVELELDGERVALPSLEGIIVLNIGYWGGG CRLWEGMGDETYPLARHDDGLLEVVGVYGSFHCAQ IQVKLANPFRIGQAHTVRLILKCSMMPMQVDGEPW AQGPCTVTITHKTHAMMLYFSGEQTDDDISSTSDQ EDIKATE |
Expression Region |
1-567aa |
Sequence Info |
Full Length |
Source |
in vitro E.coli expression system |
Tag Info |
N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged |
MW |
83.9 kDa |
Alternative Name(s) |
Diglyceride kinase epsilon |
Relevance |
Highly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may contribute to the synthesis of phospholipids with defined fatty acid composition. |
Reference |
"Functional specialization of beta-arrestin interactions revealed by proteomic analysis." Xiao K., McClatchy D.B., Shukla A.K., Zhao Y., Chen M., Shenoy S.K., Yates J.R., Lefkowitz R.J. Proc. Natl. Acad. Sci. U.S.A. 104:12011-12016(2007) |
Purity |
Greater than 85% as determined by SDS-PAGE. |
Storage Buffer |
Tris-based buffer, 50% glycerol |
Storage |
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C. |
Notes |
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week. |
Function |
Highly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may contribute to the synthesis of phospholipids with defined fatty acid composition. |
Involvement in disease |
Nephrotic syndrome 7 (NPHS7); Hemolytic uremic syndrome atypical 7 (AHUS7) |
Subcellular Location |
Membrane, Multi-pass membrane protein, Membrane, Cytoplasm |
Protein Families |
Eukaryotic diacylglycerol kinase family |
Tissue Specificity |
Expressed predominantly in testis. Expressed in endothelium, platelets and podocytes (at protein level). |
Paythway |
OxidativePhosphorylation |
Tag Information |
N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.