Item no. |
CSB-BP018072RA-10 |
Manufacturer |
Cusabio
|
Amount |
10ug |
Category |
|
Type |
Proteins Recombinant |
Format |
Liquid or Lyophilized powder |
Specific against |
other |
Conjugate/Tag |
Myc |
Purity |
Greater than 85% as determined by SDS-PAGE. |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Available |
|
Research areas |
Signal Transduction |
Target / Protein |
Pkm |
Biologically active |
Not Test |
Expression system |
Baculovirus |
Species of origin |
Rattus norvegicus (Rat) |
Uniprot ID |
P11980 |
AA Sequence |
MPKPDSEAGTAFIQTQQLHAAMADTFLEHMCRLDI DSAPITARNTGIICTIGPASRSVEMLKEMIKSGMN VARLNFSHGTHEYHAETIKNVRAATESFASDPILY RPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATL KITLDNAYMEKCDENILWLDYKNICKVVEVGSKIY VDDGLISLQVKEKGADYLVTEVENGGSLGSKKGVN LPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFI RKAADVHEVRKVLGEKGKNIKIISKIENHEGVRRF DEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIG RCNRAGKPVICATQMLESMIKKPRPTRAEGSDVAN AVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREA EAAVFHRLLFEELARASSQSTDPLEAMAMGSVEAS YKCLAAALIVLTESGRSAHQVARYRPRAPIIAVTR NPQTARQAHLYRGIFPVLCKDAVLDAWAEDVDLRV NLAMNVGKARGFFKKGDVVIVLTGWRPGSGFTNTM RVVPVP |
Tag Info |
N-terminal 10xHis-tagged and C-terminal Myc-tagged |
Expression Region |
1-531aa |
Protein length |
Full Length |
MW |
61.8 kDa |
Alternative Name(s) |
Pyruvate kinase muscle isozyme Pkm2, Pykm |
Relevance |
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation |
References |
"The M1- and M2-type isozymes of rat pyruvate kinase are produced from the same gene by alternative RNA splicing." Noguchi T., Inoue H., Tanaka T. J. Biol. Chem. 261:13807-13812(1986) |
Purity |
Greater than 85% as determined by SDS-PAGE. |
Storage |
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C. |
Notes |
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week. |
Function |
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation (By similarity). |
Subcellular Location |
Cytoplasm, Nucleus |
Protein Families |
Pyruvate kinase family |
Tag Information |
N-terminal 10xHis-tagged and C-terminal Myc-tagged |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.