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CARD FERTIUP Preincubation Medium: PM

CARD FERTIUP Preincubation Medium: PM

Item no.	KYD-002-EX
Manufacturer	Cosmobio
Amount	1 ml
Quantity options	500 ul 25 x 500 ul 5 x 500 ul 50 x 500 ul 1 ml 25 x 1 ml 5 x 1 ml 50 x 1 ml
Category	Assisted reproductive technologies Preincubation of cryopreserved spermatocytes Reagents & Chemicals Biochemicals Media
Type	Medium
Specific against	Mouse (Murine, Mus musculus)
Citations	1. Takeo, T., Hoshii, T., Kondo, Y., Toyodome, H., Arima, H., Yamamura, KI., Irie, T. and Nakagata, Biol Reprod. 78(3):546-51. 2008. 2. Takeo, T and Nakagata, N. Lab Anim. 44(2):132-7. 2010 3. Nakagata N. 2011. Methods Mol Biol. 693: 57-73
ECLASS 10.1	32160801
ECLASS 11.0	32160801
UNSPSC	12000000
Available
Manufacturer - Category	IVF & Reproductive Engineering/Mouse; IVF & Reproductive Engineering/Media & Solutions
Storage Conditions	4°C
Maker	KYD
Short Description	Save BIG (8-15%) on multipack and multipack bundles!\\CARD FERTIUP® Preincubation Medium: PM is a preculture medium that enhances mouse sperm fertility. \\Used in IVF with thawed C57BL/6 mouse sperm frozen using CARD FERTIUP® Cryoprotectant: CPA, fertilization rates of 30% or more can be expected. CARD FERTIUP® Preincubation Medium: PM also effectively promotes IVF with fresh sperm.
Description	CARD FERTIUP^® Preincubation Medium: PM is a preculture medium that enhances mouse sperm fertility. Used in IVF with thawed C57BL/6 mouse sperm frozen using CARD FERTIUP^® Cryoprotectant: CPA, fertilization rates of 30% or more can be expected. CARD FERTIUP^® Preincubation Medium: PM also effectively promotes IVF with fresh sperm. Combined use of FERTIUP^® sperm cryoprotectant and CARD FERTIUP^® Preincubation Medium: PM greatly improves the fertilization rate of frozen-thawed sperm in C57BL/6 and 129 strains, from less than 10% using conventional methods to more than 30%. Further, when coupled with our new CARD MEDIUM, fertility rates are boosted to over 80%. Thus, combined usage of the trio of CARD FERTIUP^® Cryoprotectant: CPA, CARD FERTIUP^® Preincubation Medium: PM and CARD MEDIUM provides a high and consistent rate of fertilization from frozen-thawed mouse sperm, which will allow you to efficiently recover mouse lines when needed. For information on techniques for mouse spermatozoa cryopreservation, spermatozoa thawing, in vitro fertilization and obtaining pups through embryo transfer, please refer to the CARD website.
Intended use	To stabilise and improve spermatozoa cryopreservation results and in vitro fertilization rates for laboratory mice

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

KYD-002-EX.pdf

KYD-002-EX-datasheet.pdf

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Amount: 1 ml

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Delivery expected until 1/17/2025