Citations |
1. Takeo, T., Hoshii, T., Kondo, Y., Toyodome, H., Arima, H., Yamamura, KI., Irie, T. and Nakagata, Biol Reprod. 78(3):546-51. 2008.
2. Takeo, T and Nakagata, N. Lab Anim. 44(2):132-7. 2010
3. Nakagata N. 2011. Methods Mol Biol. 693: 57-73 |
Background |
Sperm cryopreservation is a practical and cost-effective method of archiving mouse strains in a laboratory. FERTIUP(R) and CARD MEDIUM are high-performance media for mouse sperm cryopreservation, sperm preincubation and in vitro fertilization (IVF). When used in combination, FERTIUP(R) and CARD MEDIUM provide a high and stable rate of fertilization from frozen-thawed mouse sperm, which will allow you to reproduce mice as and when you need them. Notably, the FERTIUP(R) sperm cryoprotectant and sperm preincubation medium greatly improve the fertilization rate of frozen-thawed sperm in C57BL/6 and 129 strains, from less than 10% using conventional methods to more than 30%. In addition, our new CARD MEDIUM enhances the fertility rate to over 80%. Of course, it goes without saying that FERTIUP(R) and CARD MEDIUM have no reproduction toxicity of any kind. Our newly developed system for sperm cryopreservation and IVF using FERTIUP(R) and CARD MEDIUM provides superior support for the safe and reliable preservation of your valuable mouse strains. |