Bacterial Collagenase Assay Kit

Assay

-20 C

Collagenase produced by Clostridium histolyticum, isolated by Mandl et al. in 1953, has been widely used in research and clinical fields for isolating cells and digesting connective tissues. Importantly, bacterial collagenases differ from mammalian collagenases in their substrate specificity, as bacterial collagenases cleave the native collagen molecule into multiple fragments, whereas mammalian collagenases cleave the collagen molecule at a single site into two fragments. Clostridium species infection causes severe tissue necrosis leading to gas gangrene . At the infection site, collagenases from Clostridium species facilitate extensive destruction of the extracellular matrix without regulation because of the absence of bacterial collagenase inhibitors in animal and human sera, whereas mammalian collagenase activity is strictly regulated by mammalian serum inhibitors. Chondrex, Inc. provides a rapid bacterial collagenase assay kit using soluble FITC-labeled type I collagen instead of radio-labeled collagen as a substrate. This kit can be used not only for assaying collagenase activity, but also for inhibitor assays.

Culture Media, Tissue Homogenate