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Hep-CLS-1H growing culture

Hep-CLS-1H growing culture

Item no.	CLS-440197
Manufacturer	CLS Cell Lines Service
Amount	1 cell culture flask
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	other
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	HEP-CLS-1H
Available
breed	C3H/HE
gender	Female
morphology	Epithelial-like
growthProperties	Adherent
citation	Hep-CLS-1H (CLS catalog number 400197)
biosafetyLevel	1
tumorigenic	Yes, in C3H/HE mice
cultureMedium	Ham' s F12
mediumSupplements	10% FBS, 2 mM L-glutamine
subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 5 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
splitRatio	A ratio of 1:4 to 1:8 is recommended
fluidRenewal	Every 3 to 5 days
freezeMedium	CM-1 (CLS catalog number 800150)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cell culture flask

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Delivery expected until 9/27/2024

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