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NIH-3T3-ras growing culture

NIH-3T3-ras growing culture

Item no.	CLS-440100
Manufacturer	CLS Cell Lines Service
Amount	1 cell culture flask
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	other
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	NIH3T3-ras, A51, EJ-NIH/3T3, EJ-NIH-3T3
Available
breed	NIH Swiss
age	Embryo
gender	Male
cellType	Fibroblast
citation	NIH-3T3-ras (CLS catalog number 400100)
biosafetyLevel	1
tumorigenic	Yes, in NMRI mice
viruses	MAP-test: Negative.
virusSusceptibility	Polyomavirus, SV40
reverseTranscriptase	Negative
cultureMedium	DMEM
mediumSupplements	10% FBS, 2 mM L-glutamine, 4.5 g/L glucose
doublingTime	16 hours
subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add TrypleExpress (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37 Â°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. Do not allow the cells to become confluent, subculture once per week.
splitRatio	For plates (50 mm2) use an inoculum of 3 x 105 cells per plate and subculture every 3 days For 75 cm2 flasks use 4 x 105 cells per flask and subculture every 3 days
fluidRenewal	2 times per week
freezeMedium	CM-1 (CLS catalog number 800150)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cell culture flask

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Delivery expected until 9/6/2024

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