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HROC103 T0 M1 growing culture

HROC103 T0 M1 growing culture

Item no.	CLS-330802
Manufacturer	CLS Cell Lines Service
Amount	1 cell culture flask
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	other
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	HROC103
Available
age	44 years
gender	Male
ethnicity	Caucasian
morphology	Small cells in colonies
cellType	Epithelial
growthProperties	Adherent
citation	HROC103 T0 M1 (CLS catalog number 300802)
biosafetyLevel	1
ploidyStatus	Aneuploid
msiStatus	MSS
mutationalProfile	p53 mut; APC mut; K-RasG12VA, N-Raswt, H-Raswt, PIK3CAwt, B-Rafwt
cultureMedium	DMEM:Ham' s F12
mediumSupplements	10% FBS, 2 mM L-glutamine
doublingTime	30 hours
subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add TrypLE Express (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37 Â°C for 10 to 15 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
splitRatio	A ratio of 1:3 to 1:5 is recommended
seedingDensity	2 x 10^4 cells/cm^2
fluidRenewal	Every 3 to 5 days
freezingRecovery	Few days
freezeMedium	CM-ACF (CLS catalog number 800650)
amelogenin	X (Patient male, Y lost)
CSF1PO	12
D13S317	11, 12
D16S539	12, 13
D5S818	12, 13
D7S820	9
TH01	6, 9
TPOX	8
vWA	14, 15

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cell culture flask

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Delivery expected until 9/27/2024

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