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RD growing culture

RD growing culture

Item no.	CLS-330401
Manufacturer	CLS Cell Lines Service
Amount	1 cell culture flask
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	other
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	R D, RD-2, RD 2, 130T, 130-T, 130 T, TE-32, TE 32, TE32, TE 32.T, Te 32.T
Available
age	Embryo
gender	Female
ethnicity	Caucasian
cellType	Spindle cells and large multinucleated cells
growthProperties	Adherent
citation	RD (CLS catalog number 300401)
biosafetyLevel	1
isoenzymes	G6PD, B
virusSusceptibility	Poliovirus 1, vesicular stomatitis (Indiana), herpes simplex, vaccinia
reverseTranscriptase	Negative
products	Myoglobin; myosin ATPase
karyotype	2n=48
cultureMedium	DMEM
mediumSupplements	10% FBS, 2 mM L-glutamine, 4.5 g/L glucose
subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
splitRatio	A ratio of 1:2 is recommended
fluidRenewal	Every 3 to 4 days
freezeMedium	CM-1 (CLS catalog number 800150)
amelogenin	X, X
CSF1PO	10, 11
D13S317	13
D16S539	10, 11
D5S818	11
D7S820	8, 12
TH01	44629
TPOX	9
vWA	18
D3S1358	15, 17
D21S11	28, 29
D18S51	13, 18
pentaE	12
pentaD	11, 13
D8S1179	11, 15
FGA	20, 21

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cell culture flask

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Delivery expected until 8/16/2024

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