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CCF-STTG1 growing culture

CCF-STTG1 growing culture

Item no.	CLS-330388
Manufacturer	CLS Cell Lines Service
Amount	1 cell culture flask
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	other
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	CCFSTTG1, STTG1
Available
age	68 years
gender	Female
ethnicity	Caucasian
morphology	Long, bright cells
growthProperties	Adherent
citation	CCF-STTG1 (CLS catalog number 300388)
biosafetyLevel	1
antigenExpression	HLA DR (on approx. 25% of cells)
cultureMedium	RPMI 1640
mediumSupplements	10% FBS, 2 mM L-glutamine, 4.5 g/L glucose
subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
splitRatio	A ratio of 1:3 to 1:8 is recommended
seedingDensity	2 x 10^4 cells/cm^2 will result in a confluent monolayer within 4 days.
fluidRenewal	2 - 3 times per week
freezingRecovery	After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
freezeMedium	CM-1 (CLS catalog number 800150)
amelogenin	X, X
CSF1PO	12
D13S317	11, 13
D16S539	11, 12
D5S818	12, 13
D7S820	10, 11
TH01	7, 8
TPOX	8, 11
vWA	17
D3S1358	16, 17
D21S11	28, 29
D18S51	15
pentaE	10
pentaD	11, 13
D8S1179	13, 14
FGA	20, 22

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cell culture flask

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Delivery expected until 8/30/2024

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