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SK-BR-3 growing culture

SK-BR-3 growing culture

Item no.	CLS-330333
Manufacturer	CLS Cell Lines Service
Amount	1 cell culture flask
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	Human
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	SK-Br-3, Sk-Br-3, SK BR 03, SKBR-3, SKBr-3, SK-BR3, SKBr3, SkBr3, SKBR3
Available
tissue	Breast
disease	Carcinoma
metastaticSite	Pleural effusion
age	43 years
gender	Female
ethnicity	Caucasian
morphology	Epithelial-like
growthProperties	Monolayer, adherent
citation	SK-BR-3 (CLS catalog number 300333)
biosafetyLevel	1
proteinExpression	p53 positive
antigenExpression	Blood Type A; Rh+; HLA A11, Bw22(+/-), B40, B18
isoenzymes	PGM3, 1; PGM1, 1-2; ES-D, 1; AK-1, 1-2; GLO-1, 2; G6PD, B; Phenotype Frequency Product: 0.0044
tumorigenic	Yes, in nude mice; forms poorly differentiated adenocarcinoma
mutationalProfile	TP53 mut
karyotype	(P9) hypertriploid to hypotetraploid (+A, +B, +C, +E, +F, +G, -D) with abnormalities including dicentrics, acrocentric fragments, rings, secondary constrictions, large metacentrics or polycentrics and large submetacentric marker
cultureMedium	DMEM
mediumSupplements	10% FBS, 1.5 mM L-glutamine, 4.5 g/L glucose
doublingTime	30 hours
subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
splitRatio	A ratio of 1:2 to 1:4 is recommended
seedingDensity	Start culture from cryovial at 3 x 10^4 cells/cm^2; use 2 x 10^4 cells/cm^2 for continued subcultures
fluidRenewal	2 - 3 times per week
freezingRecovery	After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
freezeMedium	CM-ACF (CLS catalog number 800650)
amelogenin	X, X
CSF1PO	12
D13S317	11, 12
D16S539	9
D5S818	9, 12
D7S820	9, 12
TH01	8, 9
TPOX	8, 11
vWA	17
D3S1358	17
D21S11	30, 30.2
D18S51	10, 13
pentaE	10, 11
pentaD	9, 12
D8S1179	11, 12
FGA	20

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cell culture flask

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Delivery expected until 9/6/2024

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