« Back
Home /
MEL-CLS-3 growing culture

MEL-CLS-3 growing culture

Item no.	CLS-330293
Manufacturer	CLS Cell Lines Service
Amount	1 cell culture flask
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	other
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	MRI-H-221
Available
age	Unspecified
gender	Male
ethnicity	Caucasian
growthProperties	Adherent
citation	MEL-CLS-3 (CLS catalog number 300293)
biosafetyLevel	1
proteinExpression	p53(+)
tumorigenic	Yes, in nude mice
viruses	Negative for: Sendai, Ektromelie, Polyoma, K-Virus, Kilham, Reo 3, PVM, LCM, M.pulmonis, MVM, Theiler' s GD VII, toolan' s H-1, MHV, LDV, RCV/SDA, M-Adenovirus, B.piliformis
mutationalProfile	V600E type BRAF Mutation was determined by DNA based methods (sequencing, RT-PCR) and protein based methods (Western Blot)
cultureMedium	DMEM
mediumSupplements	10% FBS, 2 mM L-glutamine, 4.5 g/L glucose
subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add TrypleExpress (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37 Â°C for 10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 5 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
splitRatio	A ratio of 1:2 to 1:4 is recommended
seedingDensity	1 x 10^4 cells/cm^2
fluidRenewal	Every 3 days
freezingRecovery	After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 48 hours.
freezeMedium	CM-1 (CLS catalog number 800150)
amelogenin	X, Y
CSF1PO	10, 11
D13S317	11, 13
D16S539	10, 13
D5S818	11, 12
D7S820	7, 1
TH01	44629
TPOX	8
vWA	17, 18
D3S1358	16, 18
D21S11	28, 31.2
D18S51	16, 17
pentaE	14
pentaD	12, 13
D8S1179	12, 13
FGA	21, 25

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

Request product datasheet

Request safety datasheet

Simple Order Order Subscription

Amount: 1 cell culture flask

available

Delivery expected until 9/6/2024

Express Delivery: Save 3 days

Compare

Add to wishlist

Recommend by mail