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KG-1a growing culture

KG-1a growing culture

Item no.	CLS-330234
Manufacturer	CLS Cell Lines Service
Amount	1 cell culture flask
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	other
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	KG-1A, KG1A, KG1a
Available
age	59 years
gender	Male
ethnicity	Caucasian
cellType	Myeloblast
growthProperties	Suspension
citation	KG-1a (CLS catalog number 300234)
biosafetyLevel	1
antigenExpression	HLA A30, A31, B35, Cw4
isoenzymes	G6PD, B; PGM1, 1-2; PGM3, 0; ES-D, 1; Me-2, 1; AK-1, 0; GLO-1, 2
viruses	EBNA (EBNA ): negative
reverseTranscriptase	Negative
cultureMedium	IMDM
mediumSupplements	10% FBS, 4 mM L-glutamine
doublingTime	45 hours
subculturing	Transfer the cell suspension into sterile centrifuge tubes. Collect the cells by spinning down at 300xg for 3 minutes. Discard the supernatant and resuspend the pelleted cells in fresh cell culture medium. Adjust to an optimal cell density between 1 - 3 x 10^5 cells/ml. Split the cells when a maximum cell density of 1 - 2 x 10^6 cells/ml is reached.
splitRatio	A ratio of 1:2 is recommended
fluidRenewal	Every 3 days
freezingRecovery	Allow the cells to recover from the freezing process for at least 24 hours.
freezeMedium	CM-1 (CLS catalog number 800150)
amelogenin	X, Y
CSF1PO	7
D13S317	11, 12
D16S539	11
D5S818	13
D7S820	8, 1
TH01	7, 8
TPOX	7, 9
vWA	14, 19
D3S1358	15, 16
D21S11	28, 29
D18S51	10.2, 18
pentaE	7, 13
pentaD	8, 9
D8S1179	13, 14
FGA	22

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cell culture flask

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Delivery expected until 8/16/2024

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