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DU-145 growing culture

DU-145 growing culture

Item no.	CLS-330168
Manufacturer	CLS Cell Lines Service
Amount	1 cell culture flask
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	Human
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	DU145, Du-145, DU 145, DU_145, DU.145, Duke University 145
Available
tissue	Prostate
disease	Carcinoma
metastaticSite	Brain
age	69 years
gender	Male
morphology	Epithelial-like
growthProperties	Adherent
citation	DU-145 (CLS catalog number 300168)
biosafetyLevel	1
antigenExpression	Blood Type O; Rh+
isoenzymes	Me-2, 1-2; PGM3, 2; PGM1, 1; ES-D, 1; AK-1, 1; G6PD, B; GLO-1, 2; Phenotype Frequency Product: 0.0041
tumorigenic	Yes, in nude mice; forms adenocarcinoma (grade II) consistent with prostatic primary
karyotype	(P75) hypotriploid to tetraploid with abnormalities including breaks, dicentrics, minutes and large telocentric marker
cultureMedium	EMEM
mediumSupplements	10% FBS, 2 mM L-glutamine
subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium.
splitRatio	A ratio of 1:4 to 1:6 is recommended
seedingDensity	2 x 10^4 cells/cm^2 will yield in a confluent layer in about 4 days
fluidRenewal	2 - 3 times per week
freezingRecovery	After thawing, allow the cells to recover from the freezing process for at least 24 hours.
freezeMedium	CM-ACF (CLS catalog number 800650)
amelogenin	X, Y
CSF1PO	10, 11, 12
D13S317	12, 13, 14, 15
D16S539	11, 13
D5S818	10, 12, 13
D7S820	7, 10, 11
TPOX	11
vWA	17, 18
D3S1358	16, 17
D21S11	30, 33
D18S51	12
pentaE	12, 14
pentaD	9, 13
D8S1179	13, 14
FGA	22

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cell culture flask

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Delivery expected until 8/23/2024

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