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NCH644 growing culture

NCH644 growing culture

Item no.	CLS-330124
Manufacturer	CLS Cell Lines Service
Amount	1 cell culture flask
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	other
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Available
age	66 years
gender	Female
ethnicity	Caucasian
growthProperties	Spheroid culture
citation	NCH644 (CLS catalog number 300124)
biosafetyLevel	1
antigenExpression	Highly CD133 positive
tumorigenic	Yes
ploidyStatus	Aneuploid
cultureMedium	GBM-MG
subculturing	Mechanical dissociation by pipetting (5-10 times) with an Eppendorf pipette and 1000 Î¼l tips after centrifugation of the cells (10min, 300g, 10Â°C). Subculture as soon as the cell clusters have reached an average diameter of 200 Î¼m or the cell concentration has reached 1 x 10^6 cells/ml. It is recommended to distribute the cells into new flasks containing fresh medium thus diminishing the amount of dead cells and cell debris.
splitRatio	A ratio of 1:2 to 1:5 is recommended
seedingDensity	2 x 10^5 cells/ml
fluidRenewal	2 - 3 times per week
freezingRecovery	After thawing allow the cells to recover from the freezing process for at least 24-48 hours.
freezeMedium	CM-ACF (CLS catalog number 800650)
CSF1PO	12
D13S317	10, 13
D16S539	12, 13
D5S818	9, 1
D7S820	12, 13
TH01	6, 7
TPOX	8, 11
vWA	15, 19

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cell culture flask

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Delivery expected until 9/6/2024

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