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769-P growing culture

769-P growing culture

Item no.	CLS-330106
Manufacturer	CLS Cell Lines Service
Amount	1 cell culture flask
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	other
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	769P, 769-p
Available
age	63 years
gender	Female
ethnicity	Caucasian
morphology	Epithelial-like
growthProperties	Monolayer, adherent
citation	769-P (CLS catalog number 300106)
biosafetyLevel	1
tumorigenic	Yes, in immuno-suppressed hamsters and nude mice
karyotype	Hypodiploid; modal number = 45; a large submetacentric chromosome was present in all cells.
cultureMedium	RPMI 1640
mediumSupplements	10% FBS, 2 mM L-glutamine
doublingTime	35 hours
subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
splitRatio	A ratio of 1:4 to 1:12 is recommended
seedingDensity	3 x 10^4 cells/cm^2 will result in a confluent monolayer within 4 days.
fluidRenewal	2 - 3 times per week
freezingRecovery	After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 48 hours.
freezeMedium	CM-1 (CLS catalog number 800150)
amelogenin	X, X
CSF1PO	11, 12
D13S317	10, 14
D16S539	9, 13
D5S818	12
D7S820	10, 11
TH01	6, 9.3
TPOX	8, 11
vWA	18
D3S1358	16
D21S11	28, 3
D18S51	14, 17
pentaE	7, 18
pentaD	12, 16
D8S1179	12, 16
FGA	20, 22

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cell culture flask

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Delivery expected until 9/6/2024

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