OP9

Item no.	CLS-305174
Manufacturer	CLS Cell Lines Service
Amount	1 cryovial
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	Mouse
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	OP-9
Available
Manufacturers Category	Mouse cell lines
Tissue	Bone marrow, stroma
Breed	(C57BL/6 x C3H) F2-op/op
Age	Embryo
Morphology	Fibroblast-like
Growth Properties	Adherent
Biosafety Level	1
Culture Medium	AMEM
Medium Supplements	with ribonucleosides, deoxyribonucleosides, 2.2 g/L sodium bicarbonate, 20% FBS
Subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
Split Ratio	1:2 to 1:4
Fluid Renewal	2 to 3 times per week
Freeze Medium	CM-1 (Catalog number CLS-800100)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cryovial

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Delivery expected until 8/16/2024

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