H508

Item no.	CLS-305107
Manufacturer	CLS Cell Lines Service
Amount	1 cryovial
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	Human
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	NCI H508, NCIH508, H-508, NCI-H508
Available
Manufacturers Category	Intestine cancer cell lines
Tissue	Large intestine, Cecum
Disease	Cecum adenocarcinoma
Metastatic Site	Abdominal Wall
Age	55 years
Gender	Male
Ethnicity	European
Morphology	Epithelial
Growth Properties	Mixed: floating aggregates of round cells with some attached cells
Biosafety Level	1
Protein Expression	carcinoembryonic antigen (CEA) 1709 ng/mL per 106 cells per 10 days
Antigen Expression	Blood type A, Rh+, CA19-9 antigen
Tumorigenic	Yes
Culture Medium	RPMI 1640
Medium Supplements	10% FBS
Doubling Time	32 hours
Subculturing	Remove and discard culture medium. These cells grow as a mixture of floating and adherent cells. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 1.0 to 2.0 mL of Accutase solution to flask and observe cells under a microscope until the cell layer is dispersed (usually within 2 to 3 minutes). Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37?C.
Split Ratio	1:2 to 1:4
Fluid Renewal	3 times per week
Freeze Medium	CM-1 (Catalog number CLS-800100)

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Amount: 1 cryovial

available

Delivery expected until 9/20/2024