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Embryonic kidney Species:Homo sapiens Tissue:Kidney Morphology:Epithelial Properties:Adherent Cytogenic data:transformed with Adeno and SV-40 viral DNA sequences Medium:DMEM + 2 mM Glutamine + 10% FBS Subculture: 1:10 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Homo sapiens Tissue:Kidney Morphology:Epithelial Properties:Adherent Cytogenic data:transformed with Adeno and SV-40 viral DNA sequences Medium:DMEM + 2 mM Glutamine + 10% FBS Subculture: 1:10 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Kidney Morphology:Epithelial Properties:Adherent Cytogenic data:transformed with Adeno and SV-40 viral DNA sequences Medium:DMEM + 2 mM Glutamine + 10% FBS Subculture: 1:10 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Epithelial Properties:Adherent Cytogenic data:transformed with Adeno and SV-40 viral DNA sequences Medium:DMEM + 2 mM Glutamine + 10% FBS Subculture: 1:10 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Adherent Cytogenic data:transformed with Adeno and SV-40 viral DNA sequences Medium:DMEM + 2 mM Glutamine + 10% FBS Subculture: 1:10 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
transformed with Adeno and SV-40 viral DNA sequences Medium:DMEM + 2 mM Glutamine + 10% FBS Subculture: 1:10 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
DMEM + 2 mM Glutamine + 10% FBS Subculture: 1:10 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
1:10 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Hepatitis B: Negative Hepatitis C: Negative
Hepatitis C: Negative
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