Sf9

Manufacturer	CLS Cell Lines Service
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	other
Amount	1 cryovial
Item no.	CLS-604328
eClass 6.1	32160890
eClass 9.0	32160890
Available
Alias	SF9, sf9, SF-9, Sf-9, sf-9, Sf 9, Spodoptera frugiperda clone 9, Sf clone 9, IPLB-Sf-9AE, IPLB-SF-9AE, IPLB-SF-9, IPLB-Sf-9, IPLB-Sf9
Manufacturers Category	Insect cell line
Organism	Fall armyworm
Tissue	Ovary
Applications	Transfection host
Age	Pupal stage
Gender	Female
Morphology	Round, attached, epitheloid
Growth Properties	Monolayer, adherent
Biosafety Level	1
Virus Susceptibility	Baculoviruses, Autographa californica (MNPV), St. Louis encephalitis (SLE)
Culture Medium	Medium for Sf9 cells is available from different suppliers. For example Spodopan (PAN Biotech) is recommended.
Medium Supplements	Addition of 2% FBS enhances proliferation. Use cold medium for medium exchanges or seeding of cells.
Subculturing	Detachment of cells via a cell scraper is recommended. Collect the medium with detached cells after scraping in a 15ml centrifuge tube. Add about 5ml of medium to the flask and rinse the flask several times to collect any remaining cells and combine them with the rest of the cells in the tube. Centrifuge for 3 min at 300xg, remove the supernatant, resuspend the cells in fresh, cold medium and dispense into new flasks.
Split Ratio	For the first two subcultivations a ratio of 1:3 to 1:5 is recommended. In further subcultivations cells can be split at a ratio of 1:10 to 1:20
Seeding Density	1 x 10^4 cells/cm^2. Incubate between 26 to 30?C in a nontohumidified, ambient airtoregulated incubator. Use cell culture flasks with filter caps or loosen caps to allow for oxygen exchange.
Fluid Renewal	2 to 3 times per week
Freeze Medium	CM-1 (Catalog number CLS-800650)
Amelogenin	X, X
NOTE	Deutsch: Universitäre Kunden: Für den Erwerb ist ein Material Transfer Agreement oder eine Limited Use Label License auszufüllen. Kommerzielle Kunden: Für den Erwerb ist ein Material Transfer Agreement oder ein Master Supply Agreement auszufüllen. Nach eingegangener Bestellung werden ihnen alle relevanten Dokumente zugeschickt. English: University Customers: A Material Transfer Agreement or Limited Use Label License must be completed for purchase. Commercial Customers: A Material Transfer Agreement or Master Supply Agreement must be completed for purchase. After the order is received, all relevant documents will be sent to you.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cryovial

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Delivery expected until 7/19/2024

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