CHO Cells

This cell line is an optimal choice for toxicology, industrial biotechnology and bioproduction.

Chinese hamster ovary (CHO) cells are a cornerstone in the field of biotechnology and are heavily utilized in the process of CHO cell line development for the manufacture of biopharmaceuticals. These include monoclonal antibodies, recombinant antibody expression, and vaccines. The many advantages of CHO cells underscore their popularity in biomanufacturing, positioning them as a robust and versatile animal cell line with a proven track record in genetics, molecular biology, toxicity screening, nutrition, and gene expression studies.
The contribution of CHO cells to the biopharmaceutical industry is immense, with their role in the development of recombinant antibodies and monoclonal antibody production being particularly significant. Nearly 50 biotherapeutics developed using these cells have been approved in the USA and EU, which speaks to the efficacy of CHO cells and their integral role in antibody development. Their hamster origin contributes to lower susceptibility to viruses, enhancing biosafety in biomanufacturing settings and reducing batch-to-batch variation.
CHO cells are well-suited to produce proteins that undergo post-translational modifications, which is critical for therapeutic protein production. The versatility of the Chinese Hamster Ovary-derived cells is further highlighted by their fast proliferation rates and high protein expression rates of 1-5 grams per liter of culture. The ease of cultivating CHO cells and their ability to be genetically modified makes CHO cells an optimal choice for both transient and stable expression studies.
The CHO-K1 cell line, a derivative of the original Chinese hamster ovary (CHO) cells is frequently utilized in expressing recombinant proteins, especially for the production of therapeutic proteins and recombinant antibodies. They excel in producing therapeutic proteins and antibodies due to efficient post-translational modification, notably glycosylation. Researchers modify CHO-K1 cells to enhance protein expression and tailor glycosylation for specific therapies, crucial in biomedicine.
In conclusion, the Chinese hamster ovary cell line, known for its remarkable ability to mimic human post-translational modifications, is an invaluable scientific resource. Whether overcoming the difficulty of expressing challenging proteins or monoclonal antibody production, CHO cells have revolutionized the development and production of recombinant protein therapeutics. They remain pivotal in modern medicine, serving as a cornerstone for biopharmaceutical production and reflecting the advancements in biotechnology.

Monolayer, adherent

Female

1

Supplement the medium with 10% FBS

2 to 3 times per week

CM-1 (Cytion catalog number 800100)

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.
To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Our cells are provided for in vitro laboratory research purposes exclusively and are not intended for clinical or diagnostic use, nor are they to be administered to humans or used for veterinary purposes. Users must adhere to all applicable guidelines and regulations for the handling and use of these cells in a research setting.

A ratio of 1:4 to 1:8 is recommended

Deutsch:
Universitäre Kunden: Für den Erwerb ist ein Material Transfer Agreement oder eine Limited Use Label License auszufüllen.
Kommerzielle Kunden: Für den Erwerb ist ein Material Transfer Agreement oder ein Master Supply Agreement auszufüllen.
Nach eingegangener Bestellung werden ihnen alle relevanten Dokumente zugeschickt.

English:
University Customers: A Material Transfer Agreement or Limited Use Label License must be completed for purchase.
Commercial Customers: A Material Transfer Agreement or Master Supply Agreement must be completed for purchase.
After the order is received, all relevant documents will be sent to you.