Item no. |
CLS-300822 |
Manufacturer |
CLS Cell Lines Service
|
Amount |
1 cryovial |
Category |
|
Type |
Cell line |
Specific against |
Human |
Dry ice |
Yes
|
ECLASS 10.1 |
42040401 |
ECLASS 11.0 |
42040401 |
UNSPSC |
41106509 |
Available |
|
Manufacturers Category |
Intestine cancer cell lines |
Tissue |
Colon descendens, UICC IIIa |
Disease |
Primary adenocarcinoma, TNM stage T3N1M0R0L1V1, grading G3, Lk(n) + 2, ? Lk(n) 18 |
Age |
69 years |
Gender |
Male |
Ethnicity |
Caucasian |
Morphology |
Epithelial-like |
Growth Properties |
Adherent |
Biosafety Level |
1 |
Protein Expression |
?-actin, osteopontin low, Toll-like receptor (TLR) 3 moderate, TLR4 moderate, TLR7 low, TLR8 -, PTEN |
Antigen Expression |
CD326+, CD44+, CD15+, CD71+, CD73+, CD274+, CD47+, CD54+, CD95+, CD276+, CD133- , CD66acdeweak, IDO+, cFLIP+, MHC-I+, MHCIIweak after IFN-y treatment, EpCAM+ |
Tumorigenic |
Yes, in immune-suppressed nude mice |
Viruses |
Free of human pathogenic viruses SV40, JC/BK, HBV, HCV, HIV. |
Ploidy Status |
Aneuploid |
Msi Status |
MSS |
Mutational Profile |
p53G266e, APCwt, K-RasG13D, mt13, N-Raswt, H-Raswt, PIK3CAwt, B-Rafwt |
Culture Medium |
DMEM:Hams F12 |
Medium Supplements |
10% FBS, 2 mM L-glutamine |
Doubling Time |
27 hours |
Subculturing |
Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add TrypLE Express (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37 ?C for 10 to 15 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. This cell line will result in single cell suspension. |
Split Ratio |
A ratio of 1:4 to 1:8 is recommended |
Seeding Density |
2 x 10^4 cells/cm^2 |
Fluid Renewal |
Every 3 to 5 days |
Freezing Recovery |
Fast |
Freeze Medium |
|
Amelogenin |
X (Patient male, Y lost) |
NOTE |
Deutsch:Universitäre Kunden: Für den Erwerb ist ein Material Transfer Agreement oder eine Limited Use Label License auszufüllen. Kommerzielle Kunden: Für den Erwerb ist ein Material Transfer Agreement oder ein Master Supply Agreement auszufüllen.Nach eingegangener Bestellung werden ihnen alle relevanten Dokumente zugeschickt.English:University Customers: A Material Transfer Agreement or Limited Use Label License must be completed for purchase.Commercial Customers: A Material Transfer Agreement or Master Supply Agreement must be completed for purchase.After the order is received, all relevant documents will be sent to you. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.