HROC40

Item no.	CLS-300822
Manufacturer	CLS Cell Lines Service
Amount	1 cryovial
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	Human
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Available
Manufacturers Category	Intestine cancer cell lines
Tissue	Colon descendens, UICC IIIa
Disease	Primary adenocarcinoma, TNM stage T3N1M0R0L1V1, grading G3, Lk(n) + 2, ? Lk(n) 18
Age	69 years
Gender	Male
Ethnicity	Caucasian
Morphology	Epithelial-like
Growth Properties	Adherent
Biosafety Level	1
Protein Expression	?-actin, osteopontin low, Toll-like receptor (TLR) 3 moderate, TLR4 moderate, TLR7 low, TLR8 -, PTEN
Antigen Expression	CD326+, CD44+, CD15+, CD71+, CD73+, CD274+, CD47+, CD54+, CD95+, CD276+, CD133- , CD66acdeweak, IDO+, cFLIP+, MHC-I+, MHCIIweak after IFN-y treatment, EpCAM+
Tumorigenic	Yes, in immune-suppressed nude mice
Viruses	Free of human pathogenic viruses SV40, JC/BK, HBV, HCV, HIV.
Ploidy Status	Aneuploid
Msi Status	MSS
Mutational Profile	p53G266e, APCwt, K-RasG13D, mt13, N-Raswt, H-Raswt, PIK3CAwt, B-Rafwt
Culture Medium	DMEM:Hams F12
Medium Supplements	10% FBS, 2 mM L-glutamine
Doubling Time	27 hours
Subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add TrypLE Express (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37 ?C for 10 to 15 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. This cell line will result in single cell suspension.
Split Ratio	A ratio of 1:4 to 1:8 is recommended
Seeding Density	2 x 10^4 cells/cm^2
Fluid Renewal	Every 3 to 5 days
Freezing Recovery	Fast
Freeze Medium	CM-1 (Catalog number CLS-800650)
Amelogenin	X (Patient male, Y lost)
NOTE	Deutsch: Universitäre Kunden: Für den Erwerb ist ein Material Transfer Agreement oder eine Limited Use Label License auszufüllen. Kommerzielle Kunden: Für den Erwerb ist ein Material Transfer Agreement oder ein Master Supply Agreement auszufüllen. Nach eingegangener Bestellung werden ihnen alle relevanten Dokumente zugeschickt. English: University Customers: A Material Transfer Agreement or Limited Use Label License must be completed for purchase. Commercial Customers: A Material Transfer Agreement or Master Supply Agreement must be completed for purchase. After the order is received, all relevant documents will be sent to you.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cryovial

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Delivery expected until 9/27/2024

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