TF-1

Item no.	CLS-300434
Manufacturer	CLS Cell Lines Service
Amount	1 cryovial
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	Human
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	TF1, MFD-1
Available
Manufacturers Category	Leukemia cell lines
Tissue	Bone marrow
Disease	Erythroleukemia
Applications	The TF-1 cell line can be applied in various systems due to their responsiveness to multiple cytokines. They provide a good system to investigate the proliferation and differentiation of myeloid progenitor cells. Sensitive to GM-CSF, IL-3, EPO.
Age	35 years
Gender	Male
Ethnicity	Japanese
Growth Properties	Suspension
Biosafety Level	1
Receptors Expressed	TF-1 cells do not express glycophorin A or carbonyl anhydrase I.
Culture Medium	RPMI 1640
Medium Supplements	10% FBS, 2 mM L-glutamine, 4.5 g/L glucose, 10% KMG, for long-term culture: IL-3
Subculturing	Start cultures at 2 x 10^5 cells/ml and maintain between 1 x 10^5 and 1 x 10^6 cells/ml. Subculture by transferring an aliquot of the cell suspension into a new cell culture flask already containing an appropriate amount of fresh cell culture medium.
Seeding Density	\|BiggerAs\| 2 x 10^5 cells/ml
Fluid Renewal	2 to 3 times per week
Freeze Medium	CM-1 (Catalog number CLS-800100)
Amelogenin	X, Y
NOTE	Deutsch: Universitäre Kunden: Für den Erwerb ist ein Material Transfer Agreement oder eine Limited Use Label License auszufüllen. Kommerzielle Kunden: Für den Erwerb ist ein Material Transfer Agreement oder ein Master Supply Agreement auszufüllen. Nach eingegangener Bestellung werden ihnen alle relevanten Dokumente zugeschickt. English: University Customers: A Material Transfer Agreement or Limited Use Label License must be completed for purchase. Commercial Customers: A Material Transfer Agreement or Master Supply Agreement must be completed for purchase. After the order is received, all relevant documents will be sent to you.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cryovial

available

Delivery expected until 9/20/2024