Item no. |
CLS-300333 |
Manufacturer |
CLS Cell Lines Service
|
Amount |
1 cryovial |
Category |
|
Type |
Cell line |
Specific against |
Human |
Dry ice |
Yes
|
ECLASS 10.1 |
42040401 |
ECLASS 11.0 |
42040401 |
UNSPSC |
41106509 |
Alias |
SK-Br-3, Sk-Br-3, SK BR 03, SKBR-3, SKBr-3, SK-BR3, SKBr3, SkBr3, SKBR3 |
Available |
|
Manufacturers Category |
Breast cancer cell lines |
Tissue |
Breast, mammary gland |
Disease |
Invasive ductal carcinoma |
Metastatic Site |
Pleural effusion |
Age |
43 years |
Gender |
Female |
Ethnicity |
Caucasian |
Morphology |
Epithelial-like |
Growth Properties |
Monolayer, adherent |
Biosafety Level |
1 |
Protein Expression |
p53 positive |
Antigen Expression |
Blood Type A, Rh+, HLA A11, Bw22(+/-), B40, B18 |
Isoenzymes |
PGM3, 1, PGM1, 1-2, ES-D, 1, AK-1, 1-2, GLO-1, 2, G6PD, B, Phenotype Frequency Product: 0.0044 |
Tumorigenic |
Yes, in nude mice, forms poorly differentiated adenocarcinoma |
Mutational Profile |
TP53 mut |
Karyotype |
(P9) hypertriploid to hypotetraploid (+A, +B, +C, +E, +F, +G, -D) with abnormalities including dicentrics, acrocentric fragments, rings, secondary constrictions, large metacentrics or polycentrics and large submetacentric marker |
Culture Medium |
DMEM |
Medium Supplements |
10% FBS, 1.5 mM L-glutamine, 4.5 g/L glucose |
Doubling Time |
30 hours |
Subculturing |
Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. |
Split Ratio |
A ratio of 1:2 to 1:4 is recommended |
Seeding Density |
Start culture from cryovial at 3 x 10^4 cells/cm^2. Use 2 x 10^4 cells/cm^2 for continued subcultures |
Fluid Renewal |
2 to 3 times per week |
Freezing Recovery |
After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. |
Freeze Medium |
|
Amelogenin |
X, X |
NOTE |
Deutsch:Universitäre Kunden: Für den Erwerb ist ein Material Transfer Agreement oder eine Limited Use Label License auszufüllen. Kommerzielle Kunden: Für den Erwerb ist ein Material Transfer Agreement oder ein Master Supply Agreement auszufüllen.Nach eingegangener Bestellung werden ihnen alle relevanten Dokumente zugeschickt.English:University Customers: A Material Transfer Agreement or Limited Use Label License must be completed for purchase.Commercial Customers: A Material Transfer Agreement or Master Supply Agreement must be completed for purchase.After the order is received, all relevant documents will be sent to you. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.