HeLa

Item no.	CLS-300194
Manufacturer	CLS Cell Lines Service
Amount	1 cryovial
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	Human
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	HELA, Hela, He La, He-La, Henrietta Lacks cells, Helacyton gartleri
Available
Manufacturers Category	Reproductive system cancer cell lines
Tissue	Cervix
Disease	Adenocarcinoma
Applications	This cell line is an optimal choice for transfection.
Age	30 years
Gender	Female
Ethnicity	African American
Morphology	Epithelial-like
Biosafety Level	1
Isoenzymes	G6PD, A
Virus Susceptibility	Human adenovirus 3, Encephalomyocarditis virus, Human poliovirus 1, Human poliovirus 2, Human poliovirus 3
Reverse Transcriptase	Negative
Products	Keratin, Lysophosphatidylcholine (lyso-PC) induces AP-1 activity and c-jun N-terminal kinase activity (JNK1) by a protein kinase C-independent pathway
Culture Medium	EMEM
Medium Supplements	10% FBS, 2 mM L-glutamine
Doubling Time	28 to 36 hours
Subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
Split Ratio	A ratio of 1:2 to 1:6 is recommended
Seeding Density	1 x 10^4 cells/cm^2
Fluid Renewal	2 to 3 times per week
Freezing Recovery	After thawing, plate the cells at 2 to 3 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 to 48 hours.
Freeze Medium	CM-1 (Catalog number CLS-800100)
Amelogenin	X, X
NOTE	Deutsch: Universitäre Kunden: Für den Erwerb ist ein Material Transfer Agreement oder eine Limited Use Label License auszufüllen. Kommerzielle Kunden: Für den Erwerb ist ein Material Transfer Agreement oder ein Master Supply Agreement auszufüllen. Nach eingegangener Bestellung werden ihnen alle relevanten Dokumente zugeschickt. English: University Customers: A Material Transfer Agreement or Limited Use Label License must be completed for purchase. Commercial Customers: A Material Transfer Agreement or Master Supply Agreement must be completed for purchase. After the order is received, all relevant documents will be sent to you.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cryovial

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Delivery expected until 12/13/2024

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