Comparison

HeLa Cells European Partner

Item no. CLS-300194
Manufacturer CLS Cell Lines Service
Amount 1 cryovial
Category
Type Cell line
Certificate The certificate of analysis can be requested on the website or via email at info@cytion.com. Please indicate the lot number of your product in the email.
Specific against Human (Homo sapiens)
Dry ice Yes
ECLASS 10.1 42040401
ECLASS 11.0 42040401
UNSPSC 41106509
Alias HELA, Hela, He La, He-La, Henrietta Lacks cells, Helacyton gartleri
Available
Manufacturer - Applications
Transfection host
Manufacturer - Category
Reproductive system cancer cell lines
Description
HeLa cells, derived from the cervical cancer cells of Henrietta Lacks, are an immortal cell line widely employed in biomedical research. The human cell line Hela has significantly contributed to significant research advances and continues to play a pivotal role in laboratories worldwide.
In 1951, Henrietta Lacks, a young mother of five, sought medical attention at The Johns Hopkins Hospital for vaginal bleeding, where Dr. Howard Jones identified a significant malignant tumor on her cervix. At that time, the Johns Hopkins Medicine Institute was among the few institutions offering medical care to impoverished African Americans. Henrietta Lacks underwent radium treatment for her cervical cancer, the leading therapy available then. During her treatment, a biopsy was conducted, and a sample of her cancerous cells was sent to Dr. George Otto Gey's lab. Dr. Gey had been attempting to cultivate cells from cervical cancer patients of diverse backgrounds, but without success until Henrietta's cells, which were the first cells to proliferate continuously, a discovery that set them apart from all previous samples.
Henrietta Lacks' cervical carcinoma was later found to have been caused by the Human papillomavirus (HPV). HPV is a common virus that can lead to cervical cancer among other diseases. Research on HeLa cells has significantly contributed to understanding the role of HPV in cervical cancer, leading to the development of preventive HPV vaccines, which have had a profound impact on reducing the incidence of HPV-related cancers.
These extraordinary cells, termed "HeLa" cells after Henrietta Lacks' initials, have since become instrumental in medical research. They have enabled scientists to investigate cancer cell growth, the impact of various substances, and the workings of viruses, significantly contributing to medical advancements, including the development of vaccines for polio and COVID-19, without the ethical concerns of direct human experimentation.
HeLa cells are widely used for gene function studies, recombinant protein production, and gene therapy due to their high transfection efficiency and susceptibility to viral infections. They are pivotal in researching viral behaviors, including replication and pathogenesis, and have played a key role in Hepatitis B research by expressing viral proteins and aiding in the development of diagnostic tests and vaccines, thereby significantly advancing global health measures.
HeLa cells continue to be an invaluable resource for ongoing research in medicine and science. The significance of HeLa cells and other immortal cell lines cannot be overstated, as they continue to shape the field of medicine and infectious disease research, and they represent a lasting legacy of Henrietta Lacks and her contributions to scientific advancement.
Tissue
Cervix
Growth properties
Adherent
Disease
Adenocarcinoma
Age
30 years
Gender
Female
Ethnicity
African American
Morphology
Epithelial-like
Biosafety Level
1
Culture Medium
EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c)
Medium Supplements
Supplement the medium with 10% FBS
Subculturing
Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Fluid Renewal
2 to 3 times per week
Freezing Recovery
After thawing, plate the cells at 2 to 3 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 to 48 hours.
Freeze Medium
Handling of Cryopreserved Cultures

Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Sterility
Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.
To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.
Safety Precautions
When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.
Disclaimer
Our cells are provided for in vitro laboratory research purposes exclusively and are not intended for clinical or diagnostic use, nor are they to be administered to humans or used for veterinary purposes. Users must adhere to all applicable guidelines and regulations for the handling and use of these cells in a research setting.
Warranty
We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.
Amelogenin
X, X
Split Ratio
A ratio of 1:2 to 1:6 is recommended
Seeding Density
1 x 10^4 cells/cm^2
Virus Susceptibility
Human adenovirus 3, Encephalomyocarditis virus, Human poliovirus 1, Human poliovirus 2, Human poliovirus 3
Doubling Time
28 to 36 hours
Reverse Transcriptase
Negative
Products
Keratin, Lysophosphatidylcholine (lyso-PC) induces AP-1 activity and c-jun N-terminal kinase activity (JNK1) by a protein kinase C-independent pathway
Isoenzymes
G6PD, A
NOTE
Deutsch:
Universitäre Kunden: Für den Erwerb ist ein Material Transfer Agreement oder eine Limited Use Label License auszufüllen.
Kommerzielle Kunden: Für den Erwerb ist ein Material Transfer Agreement oder ein Master Supply Agreement auszufüllen.
Nach eingegangener Bestellung werden ihnen alle relevanten Dokumente zugeschickt.

English:
University Customers: A Material Transfer Agreement or Limited Use Label License must be completed for purchase.
Commercial Customers: A Material Transfer Agreement or Master Supply Agreement must be completed for purchase.
After the order is received, all relevant documents will be sent to you.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 1 cryovial
Available: In stock
available

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