Comparison

PSN-1

Item no. C0018013
Manufacturer Addexbio
Amount One Frozen vial
Category
Type Tumour Cells
Specific against Human (Homo sapiens)
Dry ice Yes
Citations 1. Barretina J, et al. The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity. Nature. 2012;483(7391):603-607. PMID: 22460905
ECLASS 10.1 32160890
ECLASS 11.0 32160890
UNSPSC 41106509
Available
Description

Disease:

Adenocarcinoma

Origin:

The human pancreatic cancer cell line PSN1 was derived from pancreatic adenocarcinoma tissue. After transplantation into nude mice the PSN1 cell line was established from the xenograft. PSN1 cells are unique in that amplification of both c-myc (50-fold) and activated c-Ki-ras (3-6 fold) with a point mutation from GGT to CGT at codon 12 are present in the same degree as in the original tumour and contain increased amounts of c-myc and c-Ki-ras transcripts. Loss of one of the two p53 alleles and a point mutation from AAG to CAG at codon 132 in the remaining allele was also observed in PSN1.

Species:

Homo sapiens, human

Tissue:

Pancreas

Morphology:

Epithelial, epithelial-like

Properties:

Adherent

Cytogenic data:

PSN1 cells are unique in that amplification of both c-myc (50-fold) and activated c-Ki-ras (3-6 fold) with a point mutation from GGT to CGT at codon 12 are present in the same degree as in the original tumour and contain increased amounts of c-myc and c-Ki-ras transcripts. Loss of one of the two p53 alleles and a point mutation from AAG to CAG at codon 132 in the remaining allele was also observed in PSN1.

Patient:

Not Specified

Medium:

AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS

Subculture:

Subculture at a ratio of 1:2 to 1:6 using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C.

Freezing Medium:

Complete growth culture medium supplemented with 5% (v/v) DMSO

Biosafety Level:

I

Sterility:

Bacteria: Negative

Yeast: Negative

Mycoplasma: Negative

Pathogens:

HIV: Negative

Hepatitis B: Negative

DNA Profile (STR):

Amelogenin: X

CSF1PO: 12

D13S317: 10

D16S539: 10, 11

D5S818: 11, 13

D7S820: 10

THO1:6

TPOX: 8, 11

vWA: 17

Citation Format:

If use of this culture results in a scientific publication, it should be cited in the publication as: PSN-1 (AddexBio C0018013)

Storage condition
LN2
Manufacturers Category
Cell Lines

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: One Frozen vial
Available: In stock
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