Disease:
Human colorectal adenocarcinoma Origin:
This line was established from a grade IV ulcerating tumour encircling the bowel of an 82 year old Caucasian female. HLA = A32, A33, B27, B35. Species:
Homo sapiens Tissue:
Colon Morphology:
Epithelial Properties:
Adherent Cytogenic data:
2n = 46 Patient:
Female, Caucasian, 82 yrs of age Medium:
AddexBio-formulated Leibovitz's L-15 Medium (C0006-01) + 10% Fetal Bovine Serum (FBS). Subculture:
1:3 to 1:6 (80-90%) using 0.25% trypsin or trypsin/EDTA, 0% CO2; 37 C Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level:
I Sterility:
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens:
HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression:
HLA A32, A33, B27, B35; blood type AB; Rh+ Isoenzymes:
ES-D, 1; G6PD, B; PEP-D, 1; PGD, A; PGM1, 1; PGM3, 1-2 DNA Profile (STR):
Amelogenin: X; CSF1PO: 9, 10; D13S317: 11, 12; D16S539: 11, 13; D5S818: 10, 14; D7S820: 9, 10; THO1: 6, 9.3; TPOX: 8; vWA: 18, 20, 21 Mutations in SW48 Cells
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: SW48 (AddexBio C0009014) Related cells by Organ:
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