LN-229

Item no.	C0005017
Manufacturer	Addexbio
Amount	One Frozen vial
Category	Oncology Cell Lines & Cell Culture Cell Lines By Organ Brain Cancer
Type	Tumour Cells
Specific against	Human (Homo sapiens)
Dry ice	Yes
Citations	1. Diserens AC, de Tribolet N, Martin-Achard A, Gaide AC, Schnegg JF, Carrel S. Characterization of an established human malignant glioma cell line: LN-18. Acta Neuropathol. 1981;53(1):21-28. PMID: 7211194
ECLASS 10.1	32160890
ECLASS 11.0	32160890
UNSPSC	41106509
Available
Description	Disease: Glioblastoma Origin: The LN-229 cell line was established in 1979 from cells taken from a patient with right frontal parieto-occipital glioblastoma. Species: Homo sapiens, human Tissue: Brain/right frontal parieto-occipital cortex Morphology: Epithelial Properties: Adherent Patient: Caucasian, female, 60 years of age Medium: AddexBio-formulated DMEM (C0003-01) + 5% FBS Subculture: Subculture at a ratio of 1:4 to 1:6 using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium: Complete growth culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 12 D13S317: 10, 11 D16S539: 12 D5S818: 11, 12 D7S820: 8, 11 THO1:9.3 TPOX: 8 vWA: 16, 19 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: LN-229 (AddexBio C0005017)
Storage condition	LN2
Manufacturers Category	Cell Lines

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