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REH cells

REH cells

Item no.	C0003031
Manufacturer	Addexbio
Amount	One Frozen vial
Category	Oncology Neuroscience Neural Lineage Markers Molecular Targets for Neuroscience Neural Development Cell Lines & Cell Culture Cell Lines By Organ Heart & Blood Cancer
Type	Tumour Cells
Specific against	Human (Homo sapiens)
Dry ice	Yes
ECLASS 10.1	32160890
ECLASS 11.0	32160890
UNSPSC	41106509
Available
Biosafety Level	I
Citation Format	If use of this culture results in a scientific publication, it should be cited in the publication as: REH (AddexBio C0003031)
DNA Profile	Amelogenin: X, CSF1PO: 12, 13, D13S317: 11, 14, 10, D16S539: 9, 13, D5S818:11, 12, D7S820: 9, 12, THO1:7, 9, TPOX: 8, 8, vWA: 14, 15, 16
Disease	B cell precursor leukemia
Freezing Medium	Complete growth culture medium supplemented with 20% FBS; 10% (v/v) DMSO
Medium	AddexBio-formulated RPMI-1640 (C0004-01) + 10-20% FBS
Morphology	Small, round, single cells or cell clusters in suspension
Origin	established from the peripheral blood of a 15-year-old North African girl with acute lymphoblastic leukemia (ALL at first relapse) in 1973; carries t(12; 21) leading to ETV6-RUNX1 (TEL-AML1) fusion gene.
Pathogens	HIV: Negative, Hepatitis B: Negative
Patient	Black, female, 15 years of age.
Properties	Suspension
Sterility	Bacteria: Negative. Yeast: Negative. Mycoplasma: Negative
Subculture	Initially after thawing cells may be difficult to culture! maintain at high density (>2 x 10^6 cells/ml); cells grow slowly at this stage (with doubling time at 50-70 hours); minimum density at ca. 0.5 x 10^6 cells/ml; seed out at ca. 2 x 10^6 cells/ml with initially 20% FBS in a 24-well plate; after thawing viability decreases during the first several days, but cells recover afterwards; do not change medium during this time. Masimum density at 3-5x10^6 cells/mL. Culture at 5% CO2, 37C.
Tissue	Peripheral blood
Storage condition	LN2
Manufacturers Category	Cell Lines

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: One Frozen vial

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Delivery expected until 3/14/2025

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