SU-DHL-1 cells

Disease:

Large cell lymphoma; diffuse histiocytic lymphoma

Origin:

The SU-DHL-1 cell line was derived derived from Metastatic Site: peritoneal effusion

Species:

Human, Homo sapiens

Tissue:

Lymph node

Morphology:

Lymphoblast-like; histiocytic

Properties:

Suspension

Cytogenic data:

88 chromosomes; t(2; 5)(p23; q35) translocation

Patient:

Male, Caucasian, 10 years of age

Medium:

AddexBio-formulated RPMI-1640 Medium (C0004-01) + 10% FBS

Subculture:

Cultures can be maintained by the addition of fresh medium. An inoculum of 8.0 x 10^4 to 2.0 x 10^5cells/mL is recommended. Subculture when the cell concentration is between 1.0 x 10^6 and 1.5 x 10^6 cells/mL. A subcultivation ratio of 1:4 to 1:12 is recommended. Culture at 5% CO2, 37C. Feed cells once every 2 to 3 days.

Freezing Medium:

Complete culture medium supplemented with 20% FBS and 10% (v/v) DMSO