FO cells

Disease:

Plasmacytoma; myeloma

Origin:

The line was made by self fusing Sp2/0 myeloma cells using Sendai virus.

Species:

Mus musculus, mouse

Tissue:

B lymphoblast

Morphology:

Lymphoblast

Properties:

Suspension, tendency to stick on the flask

Uses:

The line can be used as a fusion partner for making B cell hybridomas. The cells are resistant to 8-azaguanine and do not produce immunoglobulin.

Patient:

Mouse

Medium:

DMEM Medium + 10% FBS

Subculture:

Cultures can be maintained by adding fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 0.5 to 1 X 10^5 viable cells/mL. Change medium every 2-3 days. Maintain cell density below 10^6 viable cells/mL. Culture at 5% CO₂; 37C.

Freezing Medium:

Complete culture medium supplemented with 10% (v/v) DMSO