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SD-1 cells

SD-1 cells

Item no.	C0003015
Manufacturer	Addexbio
Amount	One Frozen vial
Category	Oncology Cell Lines & Cell Culture Cell Lines By Organ Heart & Blood Cancer
Type	Tumour Cells
Specific against	Human (Homo sapiens)
Dry ice	Yes
Citations	1.Dhut S, Gibbons B, Chaplin T, Young BD.Establishment of a lymphoblastoid cell line, SD-1, expressing the p190 bcr-abl chimaeric protein.Leukemia 1991; 5 ( 1 ): 49-55. http://www.ncbi.nlm.nih.gov/pubmed/1847983
ECLASS 10.1	32160890
ECLASS 11.0	32160890
UNSPSC	41106509
Available
Biosafety Level	I
Cytogenic data	Human near-tetraploid karyotype - 92(88-92)<4n>XXXX, t(9; 22)(q34; q11)x2 - carries two balanced Ph translocations - tetraploid derivate of original diploid karyotype
Disease	Acute lymphoblastic leukemia
Freezing Medium	Complete culture medium supplemented with 10% (v/v) DMSO
Medium	RPMI-1640 Medium + 20% FBS
Morphology	Ovoid
Origin	Established at diagnosis of a woman with acute lymphoblastic leukemia (pre B-ALL). Cells were immortalized with EBV.
Pathogens	HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Patient	Female, 68 years of age
Properties	Ovoid to round cells growing in suspension, singly or in clumps
Sterility	Bacteria: Negative Yeast: Negative Mycoplasma: Negative
Storage	LN2
Subculture	Maintain at 0.3-1.0 x 106 cells/ml; feed cells twice-weekly, split of ca. 1:3 to 1:5; seed out at ca. 0.3 x 106 cells/ml. Although the cell line is positive for EBV (HHV-4), expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative. Culture at 5% CO2; 37° C
Tissue	Peripheral blood
Manufacturers Category	Cell Lines

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: One Frozen vial

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Delivery expected until 3/14/2025