RS4, 11 cells

Disease:

Acute lymphoblastic leukemia; B cell precursor leukemia

Origin:

Established from the bone marrow of a 32-year-old woman with acute lymphoblastic leukemia (ALL L2) in first relapse

Species:

Homo sapiens

Tissue:

Bone marrow

Morphology:

Lymphoblast

Properties:

Suspension; single, relatively small, round cells

Cytogenic data:

human hyperdiploid karyotype - 47/48< 2n> X/XX, +8, +18, t(4; 11)(q21; q23), i(7q) - sideline without +8/18 - carries MLL-AFF1 (MLL-AF4) fusion gene - resembles published karyotype

Patient:

Female, Caucasian, 32 yrs of age

Medium:

RPMI-1640 Medium + 10% FBS

Subculture:

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 0.5 X 10^6 viable cells/mL. Split saturated culture 1:3 to 1:4 every 3-4 days; Maximal density of about 4.0 x 10^6 cells/ml; 5% CO2; 37C

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO