SU-DHL-6 cells

Disease:

large cell lymphoma; diffuse mixed histiocytic and lymphocytic lymphoma; follicular B cell lymphoma; B cell lymphoma

Origin:

Established from the peritoneal effusion of a 43-year-old man with B-cell non-Hodgkin lymphoma (B-NHL), described at the time as diffuse, mixed small and large cell type; cell line carries EZH2 Y641N mutation; assigned to GCB-like lymphoma subtype (germinal center B-cell)

Species:

Homo sapiens

Tissue:

Peritoneal effusion

Morphology:

Lymphoblast-like

Properties:

Small single cells and in clusters, in suspension

Cytogenic data:

Human hyperdiploid karyotype with 2% polyploidy - 47(42-48)< 2n> X, -Y, +6, +7, del(4)(q23), del(6)(p21.3p22.2), i(6p), del(7)(q?22q?32), der(8)t(8; 9)(q24; p13), der(9)t(8; 19; 9)(q24; q13; p13), dup(11)(q24q25), t(14; 18)(q32; q21), der(22)t(?7; 22)(?q32; p11) - sideline with dic(8; 9)(q24; p13), ider(8)(q10)t(8; 9(q24; p13) - matches published karyotype - carries t(14; 18) effecting IGH-BCL2 fusion

Patient:

Male, Caucasian, 43 yrs of age

Medium:

RPMI-1640 Medium + 10% FBS

Subculture:

Cultures can be maintained by the addition of fresh medium. Seed cells at 8 X 104 to 3 x 10^5 cells/mL is recommended. Subculture when cell concentration is between 7 x 105 and 1 x 10^6 cells/mL; a certain amount of cell debris is always visible; A subcultivation ratio of 1:3 to 1:4 is recommended.5% CO2; 37C

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO