« Back
Home /
Native Porcine Pancreatic Elastase-1, High Purity

Native Porcine Pancreatic Elastase-1, High Purity

Item no.	CS-CRP262B
Manufacturer	Cell Sciences
Amount	1000 Units
Category	Oncology Peptides & Proteins Cardiovascular Research Atherosclerosis Enzymes By Organ Pancreatic Cancer
Type	Proteins
Specific against	Pig (Porcine, Sus scrofa domesticus)
Host	Porcine
Purity	Homogenous on 15% polyacrylamide electrophoresis at pH 4.5 and on gel exclusion chromatography (G200) at pH 5.0 and 25C. Trypsin not detected on 0.5 mg elastase after more than 24 hour incubation at 25C on the substrate N-benzoyl-DL-arginine-pNA (BAPNA).
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Similar products	ELA1, Elastase-1
Available
Description	Native Pig Pancreatic Elastase
Formulation	Lyophilized from a salt free buffer. Free of trypsin, chymotrypsin, lipase, alpha-amylase, and the carboxypeptidases. This high purity elastase is isolated from 2X crystallized elastase. The 2X elastase is chromatographed on DEAE-cellulose at pH 8.8 to remove anionic non-specific protein. The elastolytic effluent is chromatographed at pH 5.0 on CM-cellulose recovering the highest specific activity by elution with salt gradient. Trace trypsin, and chymotrypsin contamination is removed by affinity chromatography.
Molecular Weight	25.5 kDa
Reconstitution	Centrifuge vial prior to opening . Reconstitute in sterile distilled water.
Storage and Stability	Stable for 1 year at 2-4C.
Specificity	115-125 units/mg of protein on the substrate elastin-orcein. One unit will solubilize 1 mg of elastin in 20 minutes at pH 8.8 and 37C. > 9 units/mg of protein on the substrate Suc-Ala-Ala-Ala-pNA. One unit will hydrolyze 1 umole per minute at pH 8.3 and 25C.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

CS-CRP262B-datasheet.pdf

Request safety datasheet

Simple Order Order Subscription

Amount: 1000 Units

available

Delivery expected until 3/14/2025