Recombinant Ag85B His

Ag85B although stable room temperature for 4 weeks, should be stored desiccated below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please note that the addition of any carrier protein into this product may produce unwanted endotoxin. This depends upon the particular application employed. Avoid repeated freeze-thaw cycles.

Antigen 85B Mycobacterium Tuberculosis-is the most abundant protein exposed by M. Tuberculosis, as well as a potent immunoprotective antigen and a leading drug target. Ag85 induces strong T-cell proliferation and IFN-g secretion in most healthy individuals exposed to M. tuberculosis, in BCG-vaccinated mice and humans, whereas the antibody against Ag85 are more prevalent in active tuberculosis patients with decreased cellular immune response. Ag85B Recombinant His-Tag fusion protein (1-325 a.a.) produced in, E. coli, is a single, non-glycosylated polypeptide chain having a MW = 30kDa.

Centrifuge vial prior to opening. First add sterile water to the vial to fully solubilize the protein to a concentration not less than 100 ug/ml. After complete solubilization of the protein, it can be further diluted to other aqueous solutions.