Recombinant DnaK Substrate Binding Domain (aa 508-638)

Store at 4C if entire vial will be used within 2-4 weeks. Store, frozen at -20C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

DnaK, originally identified for its DNA replication by bacteriophage l in, E. coli, is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins. Dnak (residues 508-638) of the substrate binding domain is a-helical and appears to act as a lid covering the substrate binding cleft. DnaK (amino acid 508-638) was purified to apparent homogeneity by using conventional column chromatography techniques. Additional amino acid (Met) is attached at N- terminus. Recombinant DnaK Lid Covering Substrate domain produced in, E. coli, is a single, non-glycosylated polypeptide chain containing 132 amino acids.