Recombinant E. coli DNAK ATPase Binding domain (aa 1-384)

Store at 4C if entire vial will be used within 2-4 weeks. Store, frozen at -20C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

DnaK, originally identified for its DNA replication by bacteriophage l in, E. coli, is the bacterial HSP-80 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins. DnaK (amino acids1-384) is N-terminal ATPase domain and ATP bound to the ATPase domain induces a conformational change in the substrate binding domain (residues 385-638). The protein coding region of the ATPase domain of DNAK (amino acids 1-384) was amplified by PCR and cloned into an, E. coli, expression vector. The ATPase domain of DNAK was purified to apparent homogeneity by using conventional column chromatography techniques. Recombinant DnaK Substrate Binding Domain produced in, E. coli, is a single, non-glycosylated polypeptide chain containing 384 amino acids.