Recombinant DNAK Substrate Binding Domain (aa 385-638)

Store at 4C if entire vial will be used within 2-4 weeks. Store, frozen at -20C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

DnaK, originally identified for its DNA replication by bacteriophage l in, E. coli, is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins. The protein coding region of the substrate binding domain of DnaK (amino acids 385-638) was amplified by PCR and cloned into an, E. coli, expression vector. The substrate binding domain of DNAK was purified to apparent homogeneity by using conventional column chromatography techniques. Additional amino acid (Met) is attached at N- terminus. Recombinant Dnak Substrate Binding Domain C-terminal produced in, E. coli, is a single, non-glycosylated polypeptide chain containing 255 amino acids.