Recombinant Human RPS6KA1/RSK1 GST Active

Recombinant full-length human RSK1 containing N-terminal GST tag was expressed by baculovirus in Sf 9 insect cells. The RSK (ribosomal S6 kinase) family comprises growth factor-regulated serine/threonine kinases, known also as p90(rsk). RSK1 contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPKsignaling pathway. The cloning and characterization of 3 genes encoding RSKs, and HU1 (also named RPS6KA1, or RSK1) cDNA encodes a predicted 735-amino aprotein containing 2 distinct consensus ATP-binding site sequences. Northern blot and RNase protection analyses detected an approximately 3.5-kb HU1 transcript in lymphocytes, skeletal muscle, liver, and adipose tissue. The expression of the RSK1, RSK2, anRSK3 genes in various human tissues, during mouse embryogenesis, and in mouse brain was determined. RSKs are implicated in the activation of the mitogen-activated kinase (MAPK) cascade and thstimulation of cell proliferation and differentiation.

Store product frozen at or below -70C. Stable for 1 year at -70C as undiluted stock. Aliquot to avoid repeated thawing and freezing.