Native Human Factor IXa *di

Recommended storage at -80C. Thaw rapidly in a 37C water bath without allowing protein to warm to 37C and immediately cool on ice. After first thaw, store in working aliquots at -80C. Stable for 3 years from date of delivery. Avoid repeated freeze-thaw cycles.

Native Human Factor IXa is prepared from Human Factor IX by activation with Bovine Factor XIa. The Bovine Factor XIa is removed after activation. Complete activation is observed by SDS-PAGE. The Factor XIa activates Factor IX in a two-step reaction. In the first step, an internal Arg-Ala bond is cleaved, and in the second step, an Arg-Val bond is cleaved. The second cleavage leads to the liberation of an activation peptide from the NH2-terminal portion of the heavy chain to produce Factor IXa-beta. Factor IXa-alpha has only the second cleavage at the Arg-Val bond with about half the coagulant activity of Human Factor IXa-beta. Complete activation is observed by SDS-PAGE. This protein purity is determined by SDS-PAGE and shows total reduction upon incubation with 2-mercaptoethanol. Factor IXa-alpha has only the second cleavage at the Arg-Val bond with about half the coagulant activity of Human Factor IXa beta.

12, 500 PEU/mg