Recombinant Human FGF1/FGF-acidic (aa 141)

Fibroblast Growth Factor acidic (FGF acidic), also known as FGF-1 and endothelial cell growth factor, is a member of the fibroblast growth factor (FGF) family. Fibroblast growth factor was found in pituitary extracts in 1973 and then tested in a bioassay that caused fibroblasts to proliferate. After further fractionating the extract using acidic and basic pH, two different forms have isolated that named “acidic fibroblast growth factor: (FGF1) and “basic fibroblast growth factor” (FGF2). Human FGF-acidic shares 54% amino acid sequence identity with FGF-basic. In mammalian FGF receptor family has 4 members, FGFR1, FGFR1, FGFR3, and FGFR4, and 1, 2, 3 have 2 sub-types “b”, “c”. aFGF can bind and activate all 7 different FGFRs. Affinity between aFGF and its receptors can be increased by heparin or heparin sulfate proteoglycan. FGF-acidic plays an important role in the regulation of cell survival, cell division, antiogenesis, cell differentiation and cell migration. FGF-acidic is also involved in a variety of biological processes, including embryonic development, morphogenesis, tissue repair, tumor growth and invasion. Recombinant Human FGF acidic is a single, non-glycosylated polypeptide chain containing 141 amino acids.

Lyophilized from a sterile filtered solution in PBS, pH 7.4

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. This depends upon the particular application employed. Further dilutions should be made in appropriate buffered solutions.

>3.3 x 10E6 IU/mg in the presence of 10 ug /ml heparin.