Rabbit Anti-Human STAT1 (CT) pAb

STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5a, Stat5b, and Stat6), Stat1, Stat3, Stat5a, and Stat5b have a wide activation profile (1, 2). STAT1 is activated by many different ligands including IFN family (IFN-a, IFN-b, IFN-g, and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1) (3). STAT1 has two forms, the 91 kDa STAT1a and the 84 kDa STAT1b, which are encoded by the same gene with splicing variant (4). This polyclonal antibody can be used for Western blot at 1:1000 to 1:2000 and for immunoprecipitation at 2 to 4 ug per sample. It is human, mouse, and rat reactive. Whole cell lysate from HeLa, Jurkat, or A431 cells can be used as positive control and a 91 kDa band can be detected to the 84 kDa STAT1b. For research use only.