Rabbit Anti-Human IL-1RAP (CT) Affinity Purified pAb

The pro-inflammatory cytokine IL-1 induced cellular response requires two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP) (1). IL-1RAcP forms a complex with IL-1RI in response to IL-1 treatment. The IL-1 receptor-associated kinase (IRAK), which mediates activation of NF-kB inducing kinase (NIK) and of NF-kB, recruits to the IL-1R complex through IL-1RAcP (2). IL-1 activation of stress-activated protein kinase and of acid sphingomyelinase also requires IL-1RAcP (3, 4). Like IL-1RI, IL-1RAcP subunit is essential for IL-1 mediated cellular response. IL-1RAcP is expressed in many tissues. This polyclonal antibody can be used for detection of IL-1RAcP by Western blot at 1:500 to 1:1000 dilution. Whole lysate from HeLa cells can be used as positive control and an approximately 66 kDa band can be detected. It has no cross activity to other members in the IL-1 receptor family. For research use only.